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Sumayao, R., Jr., Newsholme, P

Glycine

Weight:50gm

Incubation of recombinant HPV-16 E7 with cell lysate and SDS-PAGE analysis HaCaT cells were lysed in 50 mM Tris-Cl, pH 7.4 with 5 mM EDTA, 250 mM NaCl, and 0.1% Triton X-100 (lysis buffer) plus protease inhibitors (1 mM PMSF, 10 µg/ml aprotinin and leupeptin) for 30–40 min on ice